Versamax molecular devices. The final mixtures were allowed to react at 25 °C in the dark for 5 min. Versamax molecular devices

 
 The final mixtures were allowed to react at 25 °C in the dark for 5 minVersamax molecular devices  In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections

The absorbance at 570 nm was determined by VersaMax (Molecular Devices, CA) to estimate MTT-formazan production after 24, 48, and 72 hours of incubation to evaluate the proliferation rate. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements of absorbance, fluorescence, and luminescence from 6 to 384-well microplates. +81-3-5282-5261 SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Learn how to use SoftMax. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The preparation of cytoplasmic and nuclear proteins was performed by using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Invitrogen, USA) according to. Cells were lysed in NETN buffer (100 mM NaCl, 20 mM Tris pH 8. Tested w/warranty. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 0A 2 Lines Fused, unplug before ser. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial. It was verified that absorbance values were proportional to the amount of a bound peroxidase-labeled antibody. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. Concentration was determined by BCA Protein Assay Kit (ref. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. versamax, vmax, emax, uvmax, flexstation, softmax pro 5 gxp software, spectratest, molecular devices automation partners, gemini, lmax, assay protocols, data display, data analysis, assay. US HEADQUARTERS. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. 4. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax. ¨We would like to show you a description here but the site won’t allow us. The average OD 490 of 12 blank wells (Line 18) was then subtracted from the raw OD 490 obtained for each sample well. 00 Regular price. This reliable model allows for. for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. View the supported Window operating systems for the different versions of the SoftMax Pro software. ®SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 2 mM PLP and 5. Spectrofluorometer (e. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. 2. Supplier: Molecular Devices. 45% of MTT reagent in cell culture media was substituted and incubated for 4 h before adding 100 μL of 100× DMSO. ) for the next 36 h and provided a time-proliferation curve as a test result for each well of the microplate. Conventions Used in this Manual. Cell-free AB broth (22) of an overnight culture of the acylhomoserine lactone-defective V. Sale Sold out. 003 OD ±1. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 4 0112-0126B SafetyInformation InformationaboutthesafeuseoftheinstrumentfromMolecularDevices®includesan Get the most out of SoftMax Pro. The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit of <i>Terminalia chebula</i> Retzius. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes. Reboot the computer. The absorbance of the mixture was measured with a microplate reader (VERSAmax™, Molecular device, Sunnyvale, USA) at wavelength of 498 nm until absorbance was saturated. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). 00 USD. 003 OD ±1. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The optical density was measured at a wavelength of 450 nm using VersaMax (Molecular Devices, Seoul, South Korea). StakMax. Shop on LabX Shop by Category Shop by Brand Shop by Application Shop by Reseller Sell on LabX Sell Your Equipment Advertise with LabX My AccountMolecular Devices Absorbance Microplate Reader VersaMax. Conventions Used in this Manual. We would like to show you a description here but the site won’t allow us. Windows Home Editions are not supported. Measurement of biofilm formation inhibitory activity. Molecular Devices Versamax Tunable Microplate Reader is available from The Lab World Group. 滤光片型光吸收微孔板读板机(酶标仪),简单易用,性价比高,检测结果精确. Get pricing and purchase lab equipment and. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. No accessories are needed and SoftMax® Pro Software. The nuclear extracts were subsequently determined for the content of NF-κB with the NF-κB (p65) transcription factor assay kit. The system provides automation for up to 50 For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. TECHNICAL NOTE. HepG2 cells were seeded into 6-well plates. 1700 +1 800. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both. developing diagnostic and research tools using. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. Software (optional), such as Softmax Pro (Molecular Devices). The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein. In brief, mature 3T3-L1 cells were washed with DPBS, fixed with 10% formalin for 1 h at room temperature, washed once with 60%. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Absor-bance of the solution was measured using a micro plate Reader (VersaMax, Molecular Devices) at 570 nm. MDC instruments including: SpectraMax®, VersaMax. 47 shipping. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Proliferation of the released daughter cells was monitored at a wavelength of 578 nm online by a microplate reader (VersaMax; Molecular Devices, Sunnyvale, Calif. Readings were taken every 15 s at 490 nm and the results were analysed using SOFT-MaxPro 4. TECHNICAL NOTE. Select Add or Remove Programs system settings. The signal was developed using 3,3′, 5,5′-tetramethylbenzidine after streptavidin–peroxidase treatment. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). Plates values were acquired using a fluorescence microplate reader (570 nm; Versamax, Molecular Devices, San Jose, CA, United States). Primer design. 2. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. New and used Molecular Devices VersaMax's for sale on LabX. VERSAMAX - VersaMax Tunable Microplate Reader - VersaMax Tunable Microplate Reader, Molecular Devices - Each Brand: Molecular Devices $20,893. printed in u. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Sterile AB medium and a mixture of Me 2 SO, methanol, and water (ratio v/v 1:1:8) served as negative controls. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. MOLECULAR DEVICES VersaMax is a customizable lab equipment capable of performing multiple types of research-grade tests and experiments at once, with enhanced safety protocols and adjustable column and sample-loading system for efficient sample preparation and increased throughput. For more information, please visit us at 160 µL of sample solutions/positive control (ascorbic acid) were mixed with 40 µL of DPPH solution and incubated at room temperature for 30 min. Molecular Devices VersaMax Microplate Reader. Turn the reader on and secure the USB cable. VersaMax ELISA Microplate Reader from Molecular Devices, LLC Description Enables precise and accurate detection at wavelengths in the visible spectrum without the use of. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The water extract afforded the greatest yield, and total phenolic and tannin. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. A standard curve was made using the known concentrations of 4-nitrophenol (Sigma). 9. Manufacturer: Molecular Devices; Includes PC Workstation w/ Softmax Pro Software Wavelength Range (nm): 340–850 Wavelength Accuracy: < ±0. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Figure S2: The cellular localization of NDRG1 is not affected by N- or C- terminal deleted NDRG1 mutants under hypoxic conditions. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). ~ Bio-Tek ELx808 Microplate Reader $2,795. 9000-0756. Molecular Devices Beijing Tel. Absolute values were attained with a standard curve composed of type I bovine skin collagen solution (0. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. 1 M sodium phosphate buffer (pH 6. For validation of the Molecular Devices VersaMax™ Absorbance Microplate Reader for use with the Pyrochrome Assay, data acquisition and analysis were performed with SoftMax ® Pro GxP Software. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). Select up to two wavelengths. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. Molecular Devices VersaMax Absorbance (UV/Vis) Elisa Microplate Reader For Sale for Plate Reader is complete with computer, SoftMa. 0 OD. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. from Molecular Devices Corporation. The colour change of the MTS solution was measured at 490 nm by using a tunable microplate reader (VersaMax Molecular Devices, USA). ChE activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Supplier: Molecular Devices. If the computer is connected to the Internet: Select Search Automatically for. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The viability of the treated group was expressed as a percentage of non-treated control group, which was assumed to be 100%. VersaMax ELISA Microplate Reader. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机(酶标仪),具备终点法和动力学检测模式,标准配置6块滤光片,覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. Molecular Devices VersaMax Tunable Microplate Reader. Open the Windows Task Manager, go to the Services tab, and click Open Services. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). The quantity of nitrite in the samples was calculated using the concentration of sodium nitrite as a standard. , Sunnyvale, CA, USA). The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). They were then pre-treated with indicated concentration of drugs for 1 h prior to being treated or not with 5-HT or serum. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software,Get the most out of SoftMax Pro. It covers everything from installation, configuration, data acquisition, data analysis, curve fitting, to reporting and troubleshooting. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax,Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices Corporation assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. For determination of the optimal temperature, EfmTDC was incubated in 100 mM sodium acetate buffer (pH 5. 0. A microplate reader (VersaMax, Molecular Devices, USA) was used to detect the OD value at 450 nm. Products : General, Microplate Readers. 0 out of 5 $ 2,999. Cell viability was expressed as percent survival of untreated cells. absorbance readers. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. New and used Molecular Devices VersaMax's for sale on LabX. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. After washing in PBS at least 4 times to remove all extracellular dye, samples were fixed using 4 % PFA/PBS for. 파장 정확도 : < ±0. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. With optimized reagents, option to operate the. The calibration curve was fitted to a sigmoid by the four-parameter method in the SoftMax Pro software (Molecular Devices), and allowed the calculation of the concentration of native Antigen 18A. Ann Arbor, MI, USA). 5 mM EDTA, 0. Data were analyzed with GraphPad Prism. VERSAMAX. used. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. Microplate assays involved a 96-microplate reader (VERSAmax; Molecular devices, Sunnydale, California, USA) used with flat-bottomed 96-well microplates (NUNC, Sigma Aldrich, UK). molecular devices | 1311 orleans drive | sunnyvale, cA 94089 usA | email: info@moldev. 0 (Molecular Devices, Sunnyvale, CA). and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. For more sample throughput, the system can be easily integrated into a full robotic The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. After that, absornace were measured at 520 nm using using a microplate reader spectrophotometer VERSAmax (Molecular Devices). VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high-qualityspectrophotometer. The primers for the human genes GAPDH, SOD1, SOD2, CAT, GPX1, CCS, PRDX6, FOXO3, CDKN2A,. Invasion assayCells were seeded into the top chamber of a 96-well matrigel-coated plate with 8-m-pore polyethylene terephthalate membrane inserts (Corning). +1 800-635-5577. It also integrates with the powerful SoftMax Pro Software for. 4. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax microplate spectrophotometer provides rapid and sensitive measurements for a variety of analytes across a wide range of concentrations. $1,195. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Unit price / per . The average survival rates for cells exposed to dry. 3860 N First Street San Jose,CA 95134 USA +1 408. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机,拥有灵活检 测优势,无需加配额外的滤光片。 可调波长,无需滤光片,灵活 VersaMax读板机使用光栅单色器来选择 波长,其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到 With the VersaMax microplate reader, you will never have to buy another filter. ChE activity was normalized by protein concentration as determined by a Coomassie. Microplate Reader Shelf. Results are presented as the geometric mean and are derived from at least three experiments. Innovative and flexible design features enable accelerated sample preparation time and increased laboratory productivity of DNA, RNA, and protein samples. After treatment, cells were collected, lysed with the lysis buffer. VersaMax™ ELISA microplate reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 to 850 nm). The cells were seeded on the bottom of 24-well plates with sterile glass coverslips. Substrates and coenzymes provided and specified in the kit were added at 37°C after 1 min plate was read in a spectrophotometer (Versamax, Molecular Devices, San Jose, CA, USA) at 37°C every 1 min for 4 min. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. Preparation of cytoplasmic and nuclear protein. , Sunnyvale, CA, USA). The absorbance of each well was measured at λ = 490 nm with a VersaMax (Molecular Devices, USA) microplate reader. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. The SpectraTest® ABS1 Absorbance Validation. Used. pdf file (03 Dec 2022) Manufacturer: Molecular Devices; Category of Device: Laboratory Equipment, Measuring Instruments; Document: versamax Operator's Manual, File Type: PDF;Search for used versamax. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. ces at one of the following telephone numbers: 408 747-1700 800 635-5577 U. 72 MB. +44-118-944-8000. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Lihat Video. Table of Contents. 3 Methods 1. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. A. The robot-compatible VersaMax Reader is cost-effective—users never have to buy filters. 0 OD Kinetic Read Time: 9 Second Minimum Interval Temperature Uniformity ±0. SpectraMax® i3 Multi-Mode Detection Platform. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Fabrication of Microfiber-Based Strain SensorsWe would like to show you a description here but the site won’t allow us. Afterward, HUVECs were incubated with EPC-EVs, TFEB-EVs, or PBS for 24 h or 48 h. 0%, 0-2. Additionally, the VersaMax Microplate Reader can be seamlessly integrated with the StakMax® Microlate Handling System through the SoftMax Pro Software. Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. A. 10. 1 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 compliance to ensure data integrity. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747. BMG Labtech - THERMOstar. We use. SoftMax Pro 7. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. Battery Backup Uninterruptible Power Supply (UPS) And Power Conditioner For MDS Sciex Molecular Devices VERSAmax Microplate Reader Quote Request. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. ,, [28]. $199. RT-qPCR. 00. Softmax Pro 소프트웨어가 포함된 PC 워크스테이션 포함. (VersaMax, Molecular Devices, Sunnyvale, CA, US). 2. SpectraMax®, VersaMax, VMax. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. After incubation for 1 h, the medium was transferred to 96-well plates (SPL Life Sciences) in triplication and absorbance was measured by using the ELISA microplate reader (VersaMax, Molecular Devices, USA) at 490 nm. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 9. Ann Arbor, MI, USA). Molecular Devices Versamax; Molecular Devices Vmax; Molecular Devices Fmax Fluorescence; Packard Fluorocount Fluorescence; Packard Spectracount; Tecan Spectrafluor Plus Multilabel; Perkin Elmer HTS-7000 Plus Multilabel; Skatron Skanstacker 300 Washer; Berthold LB96P Luminescence;Finally, cells were incubated with 1% SDS for 2 h at RT to release the dye from the cells, and the cell proliferation rate was assessed by quantification of the amount of dye released from the cells using a spectrophotometer (VersaMax, Molecular Devices) at the wavelength of 570 nm. Our products enable scientists to improve productivity and efficiency in scientific discovery and research. 1% (w/v) naphtylethylenediamine-HCl], incubated at room temperature for 10 min, and then the absorbance at 540 nm was measured in a microplate reader (VersaMax™, Molecular. After freeze-drying, the. A. Add to Cart. HUVECs were seeded into 96-well plates at a density of 30,000 cells/well and allowed to attach for 12 h. An ELISA plate reader (VERSAmax; Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at 480 nm. The Organoid Innovation Center at Molecular Devices combines cutting-edge technologies with novel research methods to address key challenges of scaling complex 3D biological models. This. 0A Sunnyvale, CA Made in USA Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). NORTH AMERICA. Filterless. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The. The senescence-associated β -galactosidase activity (SA- β -gal) was measured using β -galactosidase Assay Kit following manufacturer's protocol. ). A. Select Update Driver. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Home - University of Victoriaimported directly into Excel (Microsoft, Redmond, WA) from the ELISA microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). Sing a Microplate Reader (VersaMAx, Molecular Devices). Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 00 Sale price $ 0. The color was allowed to develop for 20 min in dark and the reaction was stopped with 2. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements allowing for selection of up to two wavelengths at a time. Cell proliferation. Wavelength Selection. Tris base (100 μL of a 10 mM solution) was added to the wells to solubilize the bound SRB, and absorbance was then read at 515 nm on an automated microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA, USA). Experimental data analysis was performed with a one-way analysis of variance (Anova with Newman-Keuls post-test) in Prism 6. Briefly, 100 μl of cell culture medium was mixed with 100 μl of Griess reagent [equal volumes of 1% (w/v) sulfanilamide in 5% (v/v) phosphoric acid and 0. 5°C @ 37°C Well-to. , Sunnyvale, USA). The. Then the medium was removed and 200 μL of DMSO was added to each well to solubilize the formazan crystals. High-Throughput Screening. used. 키네틱 읽기 시간 : 최소 9초 간격. Regular price $ 0. 7. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. Proliferation was assessed by bromodeoxyuridine (BrdU) incorporation (Cell Signaling Technologies). Cell. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. Molecular Devices SoftMax Pro 5. measured over 60min using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA) at a wavelength of 405nm. Our specialization servicing microplate instrumentation and our focus on a narrow range of product lines allows us to provide the highest standards of service and ensure. Find Fanuc, Molecular Devices, Eagle, and GE for sale on Machinio. (Molecular Research Center, USA) according to the manufacturer’s. . Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Spectrophotometers. Molecular Devices Corporation. SpectraMax® Microplate Reader User Manual Download Page. And the VERSAmax reader is robotics-compatible. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. All the experiments were conducted at. 0A Sunnyvale, CA Made in USAVersaMax price. +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. GABase was a mixture of γ-aminobutyrate glutamate amino transferase. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. $199. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. Cell migration assay. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). Cell viability was represented by percentage values compared to a. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA was put in each well. Find regional support. Turn the reader on and secure the USB cable. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Unit price / per . 7. If you are looking for a comprehensive user guide for SoftMax Pro 7. Full size table. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. Order Now. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. This technical note is designed to provide information . Select the Driver tab. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. ® SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Approximately. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. This. The cells were treated with 15, 25, 30, and 45 nm-particles in order to compare the cytotoxicities of different sized nanoparticles on the treated cells. The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). The VersaMax Absorbance Microplate Reader allows for selection of. 8. While the cell covers about. The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). We would like to show you a description here but the site won’t allow us. (Molecular Devices, Sunnyvale, CA). 68 $. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All. harveyi BB152 was used as the AI-2-producing positive control (prepared as reported (11)). 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pm SpectraMax and VersaMax . The. 4 (Molecular Devices cat. S. 0A-Sunnyvale, CA-Made in USASpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Acetylcholinesterase activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction and normalized by protein concentration as determined by a Coomassie. Intracellular triglyceride assay. Molecular Devices memberdayakan para ilmuwan untuk memajukan diagnosis dini dan terapi yang lebih aman untuk pasien. This manual is subject to change without notice. To date, over 219 million cases and 4. We would like to show you a description here but the site won’t allow us. S. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A.